排序方式: 共有4条查询结果,搜索用时 15 毫秒
1
1.
海螵蛸对骨愈合相关基因表达的影响 总被引:13,自引:0,他引:13
为揭示中药海螵蛸在骨折愈合过程中的干预作用,了解海螵蛸的调节靶点,探索建构中药基因组学的途径.通过在大鼠胫骨打孔的方法建立单因素干扰模型,在不同时间点采用原位杂交方法对各类mRNA的变化进行动态观察骨愈合过程中Ⅰ、Ⅱ、Ⅲ型前胶原mRNA、转化生长因子(TGG)-β1 mRNA、骨形态发生蛋白(BMP)-2mRNA、血管内皮生长因子(VEGF) mRNA表达情况.结果显示,海螵蛸在骨折早期对Ⅰ、Ⅲ型前胶原mRNA、VEGFmRNA、BMP-2mRNA的表达升高,而对Ⅱ型前胶原mRNA、TGF-β1 mRNA的表达量无明显影响;但达到峰值后,Ⅱ、Ⅲ型前胶原mRNA表达水平下降,VEGFmRNA、TGF-β1mRNA表达量中后期维持于较高水平.表明海螵蛸与血管形成有关,对骨折软骨形成早期具有促进骨诱导的作用,并对成骨细胞的增殖及合成活性有较大影响. 相似文献
2.
骨内膜成骨的动物模型 总被引:5,自引:3,他引:2
目的 为了解骨内膜在骨折愈合不同阶段的功能变化及其调节机制。设计一个骨折愈合过程中骨内膜成骨的动物模型。方法 将3月龄雌性SD大鼠40只随机分成4组,每组10只,在左胫骨造成长5mm的环形骨膜缺损,用骨锉在骨膜缺损区造成长5mm的骨缺损,深达髓腔,约显露髓腔的1/6。结果 14天时裸露的贿腔已开始出现骨性关闭的征象,28天时髓腔完全由坚质骨关闭,而各时间点组的骨缺损处填充物均为颜痕样结缔组织。结论 相似文献
3.
ObjectiveThe aim of this study was to compare the microstructure of blood vessels and nerve fibers between different acupoints in the rat skin from the perspective of histology.MethodsThe skin tissue at “Qūzé” (曲泽 PC3)” “Dàlíng (大陵 PC7)” and “Zhōngchōng (中冲 PC9)” regions was taken from the rat forelimb along the pericardium meridian, and cut sagittally with a freezing microtome. After that, the skin sections were stained by fluorescent histochemistry and immunohistochemistry with phalloidin and calcitonin gene-related peptide (CGRP) to reveal the microstructure of blood vessels and nerve fibers respectively, and then examined under a laser scanning confocal microscope.ResultsThe microstructure of blood vessels and nerve fibers was clearly labeled with phalloidin and CGRP, respectively. The blood vessels and nerve fibers were observed in the dermis of “PC3” “PC7” and “PC9” regions, where the bundle of nerve fibers distribute in parallel to the blood vessels, and sent out thin branches to surround the wall of the blood vessels. In contrast, the density of blood vessels and nerve fibers in “PC3” “PC7” and “PC9” were presented orderly in an increased tendency.ConclusionThe results of present study demonstrate the differences of the neurovascular microstructure among “PC3” “PC7” and “PC9” in the rat, providing a histological view to insight into the specificity of different acupoints. 相似文献
4.
1